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UTHSC Assistant Professor Candidate Department of Physiology Seminar Invite with Dr. Bhairab Singh Thursday, February 17, 2022 at 2:00 PM



Thursday, February 17, 2022

Assistant Professor Candidate Guest Speaker:

Bhairab N. Singh, PhD

Assistant Professor

Department of Pediatrics

Research Scientist

Department of Biomedical Engineering

University of Minnesota


“Molecular Regulation of Mammalian Heart Regeneration”


The adult human heart has limited potential to regenerate. Previously, we showed the conserved GLI1/MYCN cascade in heart regeneration from newt to human. Further, networks involved in the proliferation-maturation transition of the human cardiomyocytes are lacking, hence, limiting the development of regenerative therapies. We have identified the Hh-GLI2-CKS1B cascade, as a regulator of the transition between proliferation and maturation in hiPSC-CMs. Gene Concept Network (GCN) analysis revealed enrichment of oxygen-rich metabolic cascade with reduced proliferation in early-to-late-stage hiPSC-CMs and in neonatal to adult mouse CM. Relative to early-stage CM, GLI-members are downregulated in late-stage CM. Inhibition of Hh- or GLI-proteins in early-stage CM decreased proliferation and enhanced CM maturation. Further, we showed GLI2 binds the promoter region and regulates CKS1B expression in hiPSC-CMs. Overexpression of CKS1B in late-stage hiPSC-CMs using modified mRNA increased proliferation; surprisingly, under the same conditions maturation features were reduced. These data establish the GLI2-CKS1B network as a toggle between proliferation and maturation in CM and as a . Analysis of the ischemic (HF) and dilated-cardiomyopathy (DCM) patients, we observed significant and exclusive enrichment of GLI2-factor relative to the non-failing heart. These findings advance our mechanistic understanding of regenerative medicine for human heart disease.


2:00 PM

Please send an email to Charles Hicks (chicks20@uthsc.edu) if you are interested in the seminar.