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Advanced Imaging Core (AIC): Zeiss Elyra 7 super-resolution microscope

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Dear UTHSC Researchers,

DO YOU NEED HIGH-RESOLUTION IMAGES TO SUPPORT YOUR PUBLICATIONS AND GRANTS?

BENEFITS:

With the AIC’s Zeiss Elyra 7 super-resolution microscope (SRM) system featuring Structured Illumination Microscopy (SIM), your lab can double the resolution of confocal microscopy with minimal changes in reagents without making significant changes to your staining protocols.

SAMPLE REQUIREMENTS-SIMILAR BUT DIFFERENT THAN CONFOCAL IMAGING

  • Coverslips must be #1.5H size thickness and made with high precision glass, features required to achieve “super resolution” precision images on the Elyra 7. **The AIC has coverglass samples available for your lab to test that are known to work on our system, please inquire**
  • All mounting media must have a RI of >1.4. The AIC recommends Vectashield (Vector Laboratories) and ProLong (Invitrogen/Life Tech) hardening media product lines.
  • All common fluorescent proteins and dyes are suitable for SIM, provided that they are compatible with the following available laser lines: 405, 488, 561, and 642nm.
  • Since the final quality of the reconstructed SIM image is dependent on the signal-to-noise ratio, samples that have been highly optimized previously to have low background with strong fluorescent labeling will yield the best images.

Book via the UTHSC iLab website.

For more information on equipment specifications and core use policies, visit:

https://uthsc.edu/research/institutional-cores/advanced-imaging-core/